Detection of Detergent-sensitive Interactions Between Membrane Proteins

1:05

Handling of the Peptide and Cells

3:02

Binding of His-tagged Proteins to the HisPur Cobalt Beads

4:39

Binding of the Peptide to the His-tagged Protein Immobilized on the Beads

5:59

Elution from Cobalt Beads

7:07

Electrophoresis, Western Blotting, and Analysis of Results

7:58

Results: Use of Immunoblot Assay to Study the Interaction between Sortilin Immobilized on Cobalt Beads and myc-tagged Peptide

9:18

Conclusion