May 4th, 2018
•Pseudomonas aeruginosa infection causes significant morbidity in vulnerable hosts. The nonredundant transposon insertion mutant library of P. aeruginosa strain PA14, designated as PA14NR Set, facilitates analysis of gene functionality in numerous processes. Presented here is a protocol to generate high-quality copies of the PA14NR Set mutant library.
Tags
Vídeos Relacionados
Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells
RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
Long Term Chronic Pseudomonas aeruginosa Airway Infection in Mice
Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis
Pseudomonas aeruginosa Induced Lung Injury Model
Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants
Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection
Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados