Perforated Patch-clamp and Electrophysiological Recordings
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Results: Representative Pituitary Cell Current Clamp Recordings
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Conclusion
TRANSCRIÇÃO
This method can help answering key questions in the field of neuroendocrinology. Such as how do pituitary cells communicate, respond to releasing factor from the brain? The main advantage of this technique is that compared to this first primary ce
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The article describes an optimized protocol for making viable brain-pituitary tissue slices, using the teleost fish medaka (Oryzias latipes), followed by electrophysiological recordings of pituitary cells using the patch-clamp technique with the perforated patch configuration.