Labeling of Olfactory Receptor Neurons with Fluorescent Calcium Indicators
2:29
Preparation of Tadpoles for Live Imaging of Presynaptic Responses
4:09
Live Imaging of Presynaptic Ca2+ Changes in Olfactory Glomeruli
4:47
Olfactory-guided Behavior Assay
5:43
Results: Tracking Olfactory-guided Behavior
7:04
Conclusion
TRANSCRIÇÃO
This method can be useful to answer key questions in neurobiology fields such as how do presynaptic terminals function in vivo. The main advantage of this set of techniques is that we show complimentary approaches to asses the function of olfactor
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Xenopus tadpoles offer a unique platform to investigate the function of the nervous system in vivo. We describe methodologies to evaluate the processing of olfactory information in living Xenopus larvae in normal rearing conditions or after injury.