Inducing Mitophagy using CCCP Treatment and Preparing FACS Samples
4:29
FACS Analysis of Mitophagy
7:16
Results: Analysis of CCCP-induced Mitophagy in HeLa-mt-Keima Cells by Flow Cytometry
7:55
Conclusion
Transcrição
The overall goal of this procedure is to provide a sensitive technique for measuring cellular mitophagy activity using flow cytometry. Here we demonstrate that this method was successfully used to assess the dramatic increase in HeLa cell mitophag
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Mitophagy, the selective degradation of mitochondria, has been implicated in mitochondrial homeostasis and is deregulated in various human diseases. However, convenient experimental methods for measuring mitophagy activity are very limited. Here, we provide a sensitive assay for measuring mitophagy activity using flow cytometry.