Results: Dissociated Medaka Pituitary Cell Viability in Primary Cell Culture
7:07
Conclusion
TRANSCRIÇÃO
This method can help answer key questions in the field of endocrinology regarding how pituitary cells function and interact in a controlled environment. The main advantages of this technique are that it promotes physiologically meaningful results
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Here we describe a protocol to prepare and maintain primary pituitary cell cultures from medaka (Oryzias latipes). The optimized conditions in this protocol take important parameters such as temperature, osmolality, and pH into consideration by mimicking the physiological conditions of the fish, thereby enabling physiologically more meaningful results.