Results: Representative Recordings from En Bloc and Slice Preparations
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Conclusion
Transcrição
This method facilitates the reproducible dissection and slicing of rodent brainstems for the captured analysis of the Mirian Medullary Respiratory Network. This technique offers great experimental flexibility, making it possible to prepare either
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This protocol both visually communicates the brainstem-spinal cord preparation and clarifies the preparation of brainstem transverse slices in a comprehensive step-by-step manner. It was designed to increase reproducibility and enhance the likelihood of obtaining viable, long lasting, rhythmically-active slices for recording neural output from the respiratory regions of the brainstem.