Prepare the Solutions for Standard Electrospinning and Set Up the Electrospinning Apparatus
1:41
Electrospinning
2:21
Generation of PCL Nanofiber Mats with Arrayed Holes
2:41
Expansion of 2D Nanofiber Mats with/without Arrayed Holes via Subcritical CO2 Liquid
3:38
Results: Visualization and In vivo Responses of PCL Nanofiber Scaffolds With and Without Expansion via Subcritical CO2
5:25
Conclusion
Transcrição
This protocol reported the transformation of traditional electrospun nanofibremembranes from 2D to 3D, via depressurization of subcritical CO2 fluid which was not realized previously. This method eliminates many issues associated with previous app
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This article demonstrates the technique of expanding a traditional, two-dimension (2D) electrospun nanofiber mat into a three-dimension (3D) scaffold through the depressurization of subcritical CO2 fluid. These augmented scaffolds are 3D, closely mimic cellular nanotopographic cues, and preserve the functions of biologic molecules encapsulated within the nanofibers.