Results: Defining the Key Parameters to Reach Highly Efficient and High Thoughput Cells (Co-) Transfections
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Conclusion
Transcrição
The NanoDispenser based transfection protocol on developed to describe in this video represents the first user-friendly, high-throughput transfection method allowing even beginners in the field to be successful. This is easy on high-throughput tec
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This protocol describes high-throughput plasmid transfection of mammalian cells in a 384-well plate using acoustic droplet ejection technology. The time-consuming, error-prone DNA dispensing and multiplexing, but also the transfection reagent dispensing, are software-driven and performed by a nanodispenser device. The cells are then seeded in these prefilled wells.