The protocol described here furnishes investigators with a method to study oral neutrophils within a ligature-induced animal model of periodontal disease in an analogous manner to human participants. The main advantage of this technique is highlig
Faça login ou inicie seu teste gratuito para acessar este conteúdo
This article presents a protocol for establishing a ligature-induced model of murine periodontitis involving multiple maxillary molars, resulting in larger areas of the involved gingival tissue and bone for subsequent analysis as well as reduced animal usage. A technique to assess oral neutrophils in a manner analogous to human subjects is also described.