Results: Peripheral Blood Mononuclear Cell (PBMC)-Derived CIK Expansion and Characterization and the Cytotoxicity Assay
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Conclusion
Transcrição
We optimized a highly qualified and clinically applicable method for the expansion of PBMC-derived cytokine-induced killer T cells and for evaluation of their cytotoxicity against cancers. The advantage of this technique is to provide a standard o
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Here, we present a protocol to perform the isolation and expansion of peripheral blood mononuclear cells-derived cytokine-induced CD3+CD56+ killer cells and illustrate their cytotoxicity effect against hematological and solid cancer cells by using an in vitro diagnosis flow cytometry system.