Only and only binding proteins control multiple biological processes. This FLAG-Biotin CLIP-seq method, hence to globally profile the special and the temporal arrangement of RNAs and RBPs in mammalian cells. This method allows efficient detection
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Here we present a modified CLIP-seq protocol called FbioCLIP-seq with FLAG-biotin tandem purification to determine the RNA targets of RNA-binding proteins (RBPs) in mammalian cells.