Tilapia lake virus is an emerging virus and contagious pathogens, that cause high mortality in tilapia and its hybrid species. As tilapia is a main protein source for many developing countries, the new virus infection has a great impact to tilapia
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We present an RT-LAMP assay for the detection of TiLV in tilapia fish using simple instruments over a relatively short period of time compared to conventional RT-PCR techniques. This protocol may help control the epidemic spread of TiLVD, especially in developing countries.