Establishment of an Oxygen-Glucose Deprivation Model and Time-Temperature Protocol
2:30
CCK-8 Viability Assay and Analysis of Flow Cytometry for Apoptosis
3:59
Mitochondrial Depolarization Assessment and Reactive Oxygen Species Assay
5:11
Measurement of Caspase 3/ Caspase 8 Activity
5:51
Results: Evaluation of Cell Viability, Apoptosis, Mitochondrial Function, and Caspase Activity
7:40
Conclusion
TRANSCRIÇÃO
The goal of the present study was to quantify the levels of cell death, following different hypothermia treatments in a human cardiomyocyte-based model. This model can be used to study the molecular mechanism of hypothermic cardioprotection, which
Sign in or start your free trial to access this content
The distinct effects of different degrees of hypothermia on myocardial protection have not been thoroughly evaluated. The goal of the present study was to quantify the levels of cell death following different hypothermia treatments in a human cardiomyocyte-based model, laying the foundation for future in-depth molecular research.