Purification of Wild Type or Mutant Form of α2β2StTS Complex
3:53
Optimized Crystallization for Wild Type and Mutant Form of the α2β2StTS Comple
6:49
Results: Purification of the α- and β -Subunits of the Tryptophan Synthase and the Wild Type and Mutant α2β2StTS Complexes
8:50
Conclusion
TRANSCRIÇÃO
This protocol to purify the enzyme tryptophan synthase from Salmonella typhimurium is fast, simple, and short. Ammonia sulfate fractionation and size exclusion chromatography make it possible to purify the wild type and mutant tryptophan synthase
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This article presents a series of consecutive methods for the expression and purification of Salmonella typhimurium tryptophan synthase comp this protocol a rapid system to purify the protein complex in a day. Covered methods are site-directed mutagenesis, protein expression in Escherichia coli, affinity chromatography, gel filtration chromatography, and crystallization.