February 2nd, 2021
•The protocol describes how to achieve site-directed modifications in the genome of Anopheles malaria mosquitoes using the φC31 system. Modifications described include both the integration and the exchange of transgenic cassettes in the genome of attP-bearing docking lines.
Vídeos Relacionados
siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts
Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow
Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
A Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells
CRISPR/Cas9 Gene Editing to Make Conditional Mutants of Human Malaria Parasite P. falciparum
Characterizing Histone Post-translational Modification Alterations in Yeast Neurodegenerative Proteinopathy Models
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados