Results: Chemical Dimerization to Induce Chromatin-associated Condensates
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Conclusion
Transcrição
This protocol describes the chemical dimerization system to induce protein condensates on telomeres. It can be easily adapted to induce condensates on other genomic loci, and is suitable for probing the formation and function of chromatin associat
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This protocol illustrates a chemically induced protein dimerization system to create condensates on chromatin. The formation of promyelocytic leukemia (PML) nuclear body on telomeres with chemical dimerizers is demonstrated. Droplet growth, dissolution, localization and composition are monitored with live cell imaging, immunofluorescence (IF) and fluorescence in situ hybridization (FISH).