Egg Surface Sterilization and Rearing Axenic Larvae
3:47
Verification of Axenic Larvae with Culture-Dependent Assays
4:40
Verification of Axenic Individuals with Culture-Independent Assays
5:32
Results: Confirmation of Efficacy of Gut Bacteria Elimination
6:07
Conclusion
Transcrição
The gut bacteria can affect multiple physiological processes of insect hosts. Preparing and maintaining axenic larvae is a powerful tool to study gut bacteria functions. The main advantages of this protocol are that plant tissue culture is used to
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To obtain an axenic insect, its egg surface is sterilized, and the hatched larva is subsequently reared using axenic leaves. This method provides an efficient way for axenic insect preparation without administering antibiotics or developing an artificial diet, which can also be applied to other leaf-eating insects.