Preparation of Footpad Tissue for Confocal Laser Scanning Microscopy
5:01
Confocal Laser Scanning Microscopy
6:37
Results: Anatomy of the Murine Hindlimb Vasculature
7:20
Conclusion
Transcrição
This protocol presents how to create a mouse foot pad gangrene model using administration of mass inhibitor and femoral artery coagulation. DiI perfusion then allows for high-resolution visualization of the foot pad vasculature. This model is clin
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The present protocol describes a unique, clinically relevant model of peripheral arterial disease that combines femoral artery and vein electrocoagulation with the administration of a nitric oxide synthase inhibitor to induce hindlimb gangrene in FVB mice. Intracardiac DiI perfusion is then used for high-resolution, three-dimensional imaging of the footpad vasculature.