Generation and Surgical Implantation of the Mammary Imaging Window (MIW)
4:13
Positioning and Maintaining Mouse on the Microscope Stage for Imaging
5:52
Set Up for 4D, Intensity-Based, Label-Free Intravital Imaging of Dynamic Cell Behavior
8:02
Fluorescence Lifetime Imaging (FLIM) of NAD(P)H
8:43
Results: Intravital Imaging of the Mammary Tumor Microenvironment
10:15
Conclusion
Transcrição
Intravital imaging allows us to observe physiological interactions within the tumor microenvironment. And by using this method, specific dynamic behaviors that are observed can be compartmentalized using local tissue features. This protocol uses o
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The intravital imaging method described here utilizes collagen second harmonic generation and endogenous fluorescence from the metabolic co-factor NAD(P)H to non-invasively segment an unlabeled tumor microenvironment into tumor, stromal, and vascular compartments for in-depth analysis of 4D intravital images.