Preparation of Samples for Flow Cytometry Analysis
3:52
Choroid Plexus Myeloid Cells Gating
6:46
Choroid Plexus T Cells Gating
7:44
Results: Myeloid and T Cells Gating Strategy to Identify Choroid Plexus Immune Subsets
9:24
Conclusion
TRANSCRIÇÃO
This protocol may be useful for addressing questions about the role of the choroid plexuses and peripheral immune cells in regulation of the brain in various contexts in mouse models. This protocol allows for in-depth quantitative and qualitative
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This study uses flow cytometry and two different gating strategies on isolated perfused mice brain choroid plexuses; this protocol identifies the main immune cell subsets that populate this brain structure.