Preparation of Cells and Cell Culture Maintenance Medium
2:56
Virus Isolation
4:38
Results: Isolation and Amplification of Viruses Using Vertebrate and Mosquito Cell Lines
5:38
Conclusion
Transcrição
This protocol is a useful approach for isolating mosquito-associated viruses and can help in further research into the distribution of pathogens related to mosquitoes and the control of mosquito-borne diseases. Using thin lines to isolate viruses
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Numerous novel virus-like sequences have been found in mosquitoes due to the extensive use of sequencing technologies. We provide an effective procedure for isolating and amplifying viruses using vertebrate and mosquito cell lines, which might serve as the basis for future studies on mosquito-associated viruses, including mosquito-borne and mosquito-specific viruses.