Placing the Samples on Cutting Tray and Setting Vibratome
4:17
Medium and Incubator Preparation During Slicing Procedure
5:07
Preparing and Mounting Slices
7:06
Changing Medium
7:51
Results: Ex Vivo Cultivation of Human Left Ventricular Myocardial Tissue for Detailed Analysis of Contraction Forces and Kinetics
10:15
Conclusion
Transcrição
Using this protocol, human left ventricular tissue slices can be cultivated ex vivo in a biomimetic chamber. The application of pre and afterloads improves the resemblance of the physiological environment. This technique allows for continuous regi
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We present a protocol for ex vivo cultivation of human ventricular myocardial tissue. It allows for detailed analysis of contraction force and kinetics, as well as the application of pre- and afterload to mimic the in vivo physiological environment more closely.