Cell Seeding, Maintenance, and Calcification Induction
2:13
Calcification Detection via Imaging
7:51
Data Analysis
10:13
Results: A Semi-Automated Method for In Vitro Calcification Determination
11:05
Conclusion
TRANSCRIÇÃO
Our method allows to measure calcification propensity of patient samples using human vascular smooth muscle cells. This will help propel research into vascular calcification disorders, assisting in the development of new therapies. This technique
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Cardiovascular disease is the leading cause of death worldwide. Vascular calcification contributes substantially to the burden of cardiovascular morbidity and mortality. This protocol describes a simple method to quantify vascular smooth muscle cell-mediated calcium precipitation in vitro by fluorescent imaging.