JoVE Logo
Centro de Recursos para Docentes

Entrar

Generation of Brown Fat-Specific Knockout Mice Using a Combined Cre-LoxP, CRISPR-Cas9, and Adeno-Associated Virus Single-Guide RNA System

DOI :

10.3791/65083-v

March 24th, 2023

March 24th, 2023

1,672 Views

1Section on Integrative Physiology and Metabolism, Research Division, Joslin Diabetes Center, Harvard Medical School

In this protocol, we describe the technical procedures to generate brown adipose tissue (BAT)-specific knockout mice leveraging a combined Cre-LoxP, CRISPR-Cas9, and adeno-associated virus (AAV) single-guide RNA (sgRNA) system. The described steps include the design of the sgRNAs, the preparation of the AAV-sgRNA particles, and the microinjection of AAV into the BAT lobes.

Tags

Brown Fat specific Knockout Mice

-- Visualizações

Vídeos Relacionados

article

Visualizing RNA Localization in Xenopus Oocytes

article

Substrate Generation for Endonucleases of CRISPR/Cas Systems

article

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

article

A Quantitative Dot Blot Assay for AAV Titration and Its Use for Functional Assessment of the Adeno-associated Virus Assembly-activating Proteins

article

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

article

Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9

article

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

article

Process Development for the Production and Purification of Adeno-Associated Virus (AAV)2 Vector using Baculovirus-Insect Cell Culture System

article

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

article

CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications

JoVE Logo

Privacidade

Termos de uso

Políticas

Pesquisa

Educação

SOBRE A JoVE

Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados