Name: mouse epidermal neural crest stem cell (epi-ncsc) cultures
Uploaded: 2008-05-09T01:00:00
Description: Watch this Scientific Journal Video about Mouse Epidermal Neural Crest Stem Cell EPI-NCSC Cultures at JoVE.com

Supported by: USPHS grant NS38500, National Institute of Neurological Disorders and Stroke, NIH, USA; Biomedical Technology Alliance of Southeastern Wisconsin, Milwauke, WI, USA; Bryon Riesch Paralysis Foundation, Milwaukee, WI, USA; Fraternal Order of Eagles, Midwest Chapter, USA; National Spinal Cord Injury Association, Milwaukee Chapter, USA; North East England Stem Cell Institute, Newcastle University, UK.

Dissection of the Bulge from Adult Mouse Whisker Follicles
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<li>We use 10 weeks to 6 months old mice. Younger mice often yield more cells. Euthanize mouse and submerge entire animal into 1:1 mixture of betadine (iodine solution) and hydrogen peroxide (available from pharmacy) for about 3 minutes. </li>
<li>Squirt entire mouse, especially the facial region, with 75% ethanol, and carry mouse to dissection microscope in laminar flow hood.</li>
<li>Dissect whisker pads, carefully avoiding cutting into hair bulbs, ...

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	<li>Sieber-Blum, M., Grim, M., YF, H. u., Szeder, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pluripotent+neural+crest+stem+cells+in+the+adult+hair+follicle.">Pluripotent neural crest stem cells in the adult hair follicle.</a> Dev. Dyn. 231, 258-269 (2004).</li><li>Sieber-Blum, M., Grim, M., Tuan, R., Grim, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+adult+hair+follicle+-+cradle+for+pluripotent+neural+crest+stem+cells.">The adult hair follicle - cradle for pluripotent neural crest stem cells.</a> Birth Defects Research (Part C). 72, 162-172 (2004).</li><li>Sieber-Blum, M., Schnell, L., Grim, M., Schneider, R., Schwab, M. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+epidermal+neural+crest+stem+cell+(EPI-NCSC)+grafts+in+the+lesioned+spinal+cord.">Characterization of epidermal neural crest stem cell (EPI-NCSC) grafts in the lesioned spinal cord.</a> Pub. 32, 67-81 (2006).</li><li>Hu, Y. F., Zhang, Z. -. J., Sieber-Blum, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+epidermal+neural+crest+stem+cell+(EPI-NCSC)+molecular+signature.">An epidermal neural crest stem cell (EPI-NCSC) molecular signature.</a> Stem Cells. 24, 2692-2702 (2006).</li></ol>

By virtue of their migratory ability, EPI-NCSC can be isolated as a highly pure population of stem cells, which can be expanded in vitro. As embryonic remnants in an adult location, EPI-NCSC are potentially attractive candidates for future cell replacement therapies, biomedical engineering and/or regenerative medicine. We have tested EPI-NCSC in a mouse model of spinal cord injury, where they show desirable traits. Through gene expression profiling by LongSAGE (www.ncbi.nlm.nih.gov/geo, series number GSE4680) we showed t...

mouse epidermal neural crest stem cell (epi-ncsc) cultures

EPI-NCSC are remnants of the embryonic neural crest in an adult location, the bulge of hair follicles. They are multipotent stem cells that have the physiological property to generate a wide array of differentiated cell types, including neurons, nerve supporting cells, smooth muscle cells, bone/cartilage cells and melanocytes. EPI-NCSC are easily accessible in the hairy skin and can be isolated as a highly pure population of stem cells. This video provides a detailed protocol for preparing mouse EPI-NCSC cultures from whisker follicles. The whisker pad of an adult mouse is removed, and whisker follicles dissected. The follicles are then cut longitudinally and subsequently transversely above and below the bulge region. The bulge is removed from the collagen capsule and placed in a culture plate. EPI-NCSC start to emigrate from the bulge explants 3 to 4 days later.

Watch this Scientific Journal Video about Mouse Epidermal Neural Crest Stem Cell EPI-NCSC Cultures at JoVE.com

Mouse Epidermal Neural Crest Stem Cell EPI-NCSC Cultures

Here we show our method to isolate mouse epidermal neural crest stem cells (EPI-NCSC). Technique involves micro-dissecting whisker follicles, isolating the bulge and placeing it into tissue culture. EPI-NCSC start to emigrate from bulge explants onto the substratum within 3 - 4 days.

Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures

EPI-NCSC are remnants of the embryonic neural crest in an adult location, the bulge of hair follicles. They are multipotent stem cells that have the ...

Research

JoVE Journal

Biology

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