biology

Biology

cancer research

Cancer Research

biological techniques

Biological Techniques

immunology

Immunology

neuroscience

Neuroscience

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jove encyclopedia of experiments

JoVE Encyclopedia of Experiments

Online video encyclopedias of advanced research experiments for scientists in academia and biotech

Research

large animal models

This collection features research methods using porcine, bovine, equine, and leporine (rabbit) models at their embryonic, neonatal, and adult stages. These techniques include ex-vivo and in-vivo studies, organ harvesting and preservation methods, cell isolation and culturing methodologies, microbial culturing, biochemical and biophysical assays, diagnostic tests, and various surgical procedures.

Large Animal Models

drosophila melanogaster (fruit fly)

This collection features research methods using the model organism Drosophila melanogaster at each stage of its life cycle to explore a wide range of physiological and behavioral questions.

Drosophila melanogaster (fruit fly)

caenorhabditis elegans (worm)

This collection features research techniques for the metazoan Caenorhabditis elegans. This nematode worm is a powerful model system due to its transparent body, defined developmental plan, robust genetic tools, and neuro-behavioral paradigms.

Caenorhabditis elegans (worm)

danio rerio (zebrafish)

This collection features research methods using the model organism Danio rerio in its embryo, larva, and adult stages to explore physiological and behavioral questions and create disease models for screening various chemicals.

Danio rerio (zebrafish)

rodent models

This collection features research methods using rodent models in embryonic, neonatal, and adult stages. The techniques include in vitro and in vivo organ perfusion, tissue and organ isolation, microbial culturing, biochemical studies, organ transplant methodologies, behavioral tests, and surgical procedures.

Rodent Models

leporine

Leporine

porcine

Porcine

bovine

Bovine

equine

Equine

cardiopulmonary block harvest from rabbit: a surgical procedure to retrieve functional heart-lung block from rabbit model

cardiopulmonary-block-harvest-from-rabbit-surgical-procedure-to

Cardiopulmonary Block Harvest from Rabbit: A Surgical Procedure to Retrieve Functional Heart-Lung Block from Rabbit Model

Source: Pacheco-Baltazar, A., et al. Isolated Lung Perfusion System in the Rabbit Model. J. Vis. Exp. (2021)
In this video, we show a procedure to ...

isolating ipe and rpe cells: a technique for obtaining ocular pigment epithelial cells from rabbit eye

isolating-ipe-rpe-cells-technique-for-obtaining-ocular-pigment

Isolating IPE and RPE Cells: A Technique for Obtaining Ocular Pigment Epithelial Cells from Rabbit Eye

Source: Bascuas, T., et al. Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy. J. Vis. ...

tracheal cannulation in rabbit model: a procedure to insert tracheal cannula into the rabbit trachea

tracheal-cannulation-rabbit-model-procedure-to-insert-tracheal

Tracheal Cannulation in Rabbit Model: A Procedure to Insert Tracheal Cannula into the Rabbit Trachea

Source: Pacheco-Baltazar A, et al. Isolated Lung Perfusion System in the Rabbit Model. J. Vis. Exp. (2021).
This video demonstrates the surgical creation ...

nictitating membrane removal: a surgical procedure to remove nictitating membrane from rabbit eye

nictitating-membrane-removal-surgical-procedure-to-remove-nictitating

Nictitating Membrane Removal: A Surgical Procedure to Remove Nictitating Membrane from Rabbit Eye

Source: Honkanen, R. A., et al. Establishment of a Severe Dry Eye Model Using Complete Dacryoadenectomy in Rabbits. J. Vis. Exp. (2020).
In this video, we ...

superior lacrimal gland removal: a surgical technique to remove the palpebral and orbital parts of superior lacrimal gland from rabbit model

superior-lacrimal-gland-removal-surgical-technique-to-remove

Superior Lacrimal Gland Removal: A Surgical Technique to Remove the Palpebral and Orbital Parts of Superior Lacrimal Gland from Rabbit Model

Source: Honkanen, R. A. et al., Establishment of a Severe Dry Eye Model Using Complete Dacryoadenectomy in Rabbits. J. Vis. Exp. (2020).
This video ...

multisystem monitoring in rabbit model: a method to assess neuro-cardiac and respiratory parameters to study the oral drug-induced triggers for  arrhythmia and seizures

multisystem-monitoring-rabbit-model-method-to-assess-neuro-cardiac

Multisystem Monitoring in Rabbit Model: A Method to Assess Neuro-Cardiac and Respiratory Parameters to Study the Oral Drug-induced Triggers for  Arrhythmia and Seizures

Source: Bosinski, C., et al. Multi-system Monitoring for Identification of Seizures, Arrhythmias and Apnea in Conscious Restrained Rabbits. J. Vis. Exp. ...

inferior lacrimal gland removal from rabbit model: a surgical procedure to remove the larger inferior tear gland from rabbit eye orbit

inferior-lacrimal-gland-removal-from-rabbit-model-surgical-procedure

Inferior Lacrimal Gland Removal From Rabbit Model: A Surgical Procedure to Remove the Larger Inferior Tear Gland From Rabbit Eye Orbit

intermittent photic stimulation in rabbit model: a technique to induce epileptic seizures and simultaneously record multisystem parameters

intermittent-photic-stimulation-rabbit-model-technique-to-induce

Intermittent Photic Stimulation in Rabbit Model: A Technique to Induce Epileptic Seizures and Simultaneously Record Multisystem Parameters

ex vivo lung perfusion: a technique for pulmonary research in a rabbit model

ex-vivo-lung-perfusion-technique-for-pulmonary-research-rabbit

Ex vivo Lung Perfusion: A Technique for Pulmonary Research in a Rabbit Model

Source: Pacheco-Baltazar, A., et al., Isolated Lung Perfusion System in the Rabbit Model. J. Vis. Exp. (2021).
This video shows an ex vivo technique for ...

prepping conscious rabbit model for simultaneous video-eeg-ecg-oximetry-capnography for experimental interventions: a procedure to implant eee-ecg electrodes and attach respiratory probes in rabbit model

prepping-conscious-rabbit-model-for-simultaneous-video-eeg-ecg

Prepping Conscious Rabbit Model for Simultaneous Video-EEG-ECG-Oximetry-Capnography for Experimental Interventions: A Procedure to Implant EEE-ECG Electrodes and Attach Respiratory Probes in Rabbit Model

Source: Bosinski, C. et al., Multi-system Monitoring for Identification of Seizures, Arrhythmias and Apnea in Conscious Restrained Rabbits. J. Vis. Exp. ...

multisystem monitoring of epileptic abnormalities in rabbit: a method for simultaneous video eeg, ecg, capnography, and oximetry recording during induced seizures

multisystem-monitoring-epileptic-abnormalities-rabbit-method-for

Multisystem Monitoring of Epileptic Abnormalities in Rabbit: A Method for Simultaneous Video EEG, ECG, Capnography, and Oximetry Recording During Induced Seizures

rabbit embryo thawing: a warming procedure to recover cryopreserved rabbit embryos for embryo transfer

rabbit-embryo-thawing-warming-procedure-to-recover-cryopreserved

Rabbit Embryo Thawing: A Warming Procedure to Recover Cryopreserved Rabbit Embryos for Embryo Transfer

Source: Garcia-Dominguez, X. et al., Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model. J. Vis. Exp. ...

tracheal intubation for mechanical ventilation: a procedure to insert endotracheal tube in trachea of rabbit model

tracheal-intubation-for-mechanical-ventilation-procedure-to-insert

Tracheal Intubation for Mechanical Ventilation: A Procedure to Insert Endotracheal Tube in Trachea of Rabbit Model

Source: Marger, L. et al, Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution ...

embryo transfer into rabbit: a procedure for laparoscopy-guided transfer of embryos into oviduct of ovulating recipient female rabbit model

embryo-transfer-into-rabbit-procedure-for-laparoscopy-guided-transfer

Embryo Transfer Into Rabbit: A Procedure for Laparoscopy-Guided Transfer of Embryos Into Oviduct of Ovulating Recipient Female Rabbit Model

generating a rabbit calvarial defect model: a surgical procedure to create round defects in the top portion of the rabbit skull to assess bone substitute materials for bone regeneration capacities

generating-rabbit-calvarial-defect-model-surgical-procedure-to-create

Generating a Rabbit Calvarial Defect Model: A Surgical Procedure to Create Round Defects in the Top Portion of the Rabbit Skull to Assess Bone Substitute Materials for Bone Regeneration Capacities

Source: Marger, L. et al., Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution ...

jugular vein graft harvest from a rabbit model: a surgical procedure to excise a segment of rabbit jugular vein for ex vivo studies

jugular-vein-graft-harvest-from-rabbit-model-surgical-procedure-to

Jugular Vein Graft Harvest From a Rabbit Model: A Surgical Procedure to Excise a Segment of Rabbit Jugular Vein for Ex Vivo Studies

Source: Nishio, H., et al. A Rabbit Venous Interposition Model Mimicking Revascularization Surgery using Vein Grafts to Assess Intimal Hyperplasia under ...

performing injection into palpebral portion of the superior lacrimal gland: a technique for inducing dry eye disease in rabbit model

performing-injection-into-palpebral-portion-superior-lacrimal-gland

Performing Injection into Palpebral Portion of the Superior Lacrimal Gland: A Technique for Inducing Dry Eye Disease in Rabbit Model

Source: Honkanen, R. A., et al. A Rabbit Model of Aqueous-Deficient Dry Eye Disease Induced by Concanavalin A Injection into the Lacrimal Glands: ...

carotid artery and jugular vein interposition: a technique to induce venous intimal hyperplasia in rabbit model

carotid-artery-jugular-vein-interposition-technique-to-induce-venous

Carotid Artery and Jugular Vein Interposition: A Technique to Induce Venous Intimal Hyperplasia in Rabbit Model

performing injection into orbital lobe of superior lacrimal gland: a technique for inducing dry eye disease in rabbit model

performing-injection-into-orbital-lobe-superior-lacrimal-gland

Performing Injection into Orbital Lobe of Superior Lacrimal Gland: A Technique for Inducing Dry Eye Disease in Rabbit Model

Source: Honkanen, R. A. et al, A Rabbit Model of Aqueous-Deficient Dry Eye Disease Induced by Concanavalin A Injection into the Lacrimal Glands: ...

generating rabbit liver carcinoma model: a surgical procedure to implant tumor tissue in left lobe of liver for induction of hepatocellular carcinoma

generating-rabbit-liver-carcinoma-model-surgical-procedure-to-implant

Generating Rabbit Liver Carcinoma Model: A Surgical Procedure to Implant Tumor Tissue in Left Lobe of Liver for Induction of Hepatocellular Carcinoma

Source: Khabbaz, R. C. et al. Development and Angiographic Use of the Rabbit VX2 Model for Liver Cancer. J. Vis. Exp. (2019)
In this video, we demonstrate ...

ultrasound-guided concanavalin a injection into inferior lacrimal gland: a technique to inoculate inferior lacrimal gland with concanavalin a to induce dry eye disease in rabbit model

ultrasound-guided-concanavalin-injection-into-inferior-lacrimal-gland

Ultrasound-Guided Concanavalin A Injection Into Inferior Lacrimal Gland: A Technique to Inoculate Inferior Lacrimal Gland With Concanavalin A to Induce Dry Eye Disease in Rabbit Model

Source: Honkanen, R. A. et al. A Rabbit Model of Aqueous-Deficient Dry Eye Disease Induced by Concanavalin A Injection into the Lacrimal Glands: ...

hepatic angiography: a procedure to visualize liver tumor vessels in a rabbit model

hepatic-angiography-procedure-to-visualize-liver-tumor-vessels-rabbit

Hepatic Angiography: A Procedure to Visualize Liver Tumor Vessels in a Rabbit Model

Source: Khabbaz, R. C., et al. Development and Angiographic Use of the Rabbit VX2 Model for Liver Cancer. J. Vis. Exp. (2019).
In this video, we ...

generating rabbit model of bone infection: a technique of inducing chronic infection by injecting bacteria directly in bone marrow

generating-rabbit-model-bone-infection-technique-inducing-chronic

Generating Rabbit Model of Bone Infection: A Technique of Inducing Chronic Infection by Injecting Bacteria Directly in Bone Marrow

Source: Zhang, Y et al., Treatment with Vancomycin Loaded Calcium Sulphate and Autogenous Bone in an Improved Rabbit Model of Bone Infection. J. Vis. Exp. ...

assessing vessel perfusion via angiography: a technique to introduce contrast agent in descending aorta to visualize blood vessel perfusion in rabbit model

assessing-vessel-perfusion-via-angiography-technique-to-introduce

Assessing Vessel Perfusion via Angiography: A Technique to Introduce Contrast Agent in Descending Aorta to Visualize Blood Vessel Perfusion in Rabbit Model

Source: Sligar, A. D et al., Preclinical Model of Hind Limb Ischemia in Diabetic Rabbits. J. Vis. Exp. (2019).
This video describes the technique for ...

rabbit embryo vitrification using cryogenic straw:  an ultra-rapid cooling technique to preserve rabbit embryos for long-term storage

rabbit-embryo-vitrification-using-cryogenic-straw-an-ultra-rapid

Rabbit Embryo Vitrification Using Cryogenic Straw:  An Ultra-Rapid Cooling Technique to Preserve Rabbit Embryos for Long-term Storage

Source: Garcia-Dominguez, X. et al. Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model. J. Vis. Exp. ...

an experimental model of peripheral ischemia: a procedure to generate peripheral ischemia via femoral artery ligation in a rabbit

an-experimental-model-peripheral-ischemia-procedure-to-generate

An Experimental Model of Peripheral Ischemia: A Procedure to Generate Peripheral Ischemia via Femoral Artery Ligation in a Rabbit

Source: Sligar, A. D., et al. Preclinical Model of Hind Limb Ischemia in Diabetic Rabbits. J. Vis. Exp. (2019).
In this video, we demonstrate a surgical ...

tracer molecule delivery in the cisterna magna: a method to deliver fluorescent tracer molecules via direct cannulation of the cisterna magna in pig

tracer-molecule-delivery-cisterna-magna-method-to-deliver-fluorescent

Tracer Molecule Delivery in the Cisterna Magna: A Method to Deliver Fluorescent Tracer Molecules via Direct Cannulation of the Cisterna Magna in Pig

Source: B&#232;chet, N. B., et al. Direct Cannula Implantation in the Cisterna Magna of Pigs. J. Vis. Exp. (2021).
This video describes the protocol for ...

isolation of ipe and rpe cells: a technique to obtain pigmented epithelial cells from isolated porcine eye

isolation-ipe-rpe-cells-technique-to-obtain-pigmented-epithelial

Isolation of IPE and RPE cells: A Technique to Obtain Pigmented Epithelial Cells from Isolated Porcine Eye

Source: Bascuas T. et al., Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy. J. Vis. ...

locating the cisterna magna in a pig model: a surgical method to access the cisterna magna in a pig model for direct cannulation

locating-cisterna-magna-pig-model-surgical-method-to-access-cisterna

Locating the Cisterna Magna in a Pig Model: A Surgical Method to Access the Cisterna Magna in a Pig Model for Direct Cannulation

Source: B&#232;chet, N. B., et al. Direct Cannula Implantation in the Cisterna Magna of Pigs. J. Vis. Exp. (2021).
In this video, we describe a method to ...

seldinger technique based pulmonary artery catheterization: a procedure to insert pulmonary artery catheter through the jugular vein in a porcine model

seldinger-technique-based-pulmonary-artery-catheterization-procedure

Seldinger Technique Based Pulmonary Artery Catheterization: A Procedure to Insert Pulmonary Artery Catheter Through the Jugular Vein in a Porcine Model

Source: Lyhne, M. D., et al. Closed Chest Biventricular Pressure-Volume Loop Recordings with Admittance Catheters in a Porcine Model J. Vis. Exp. (2021).
...

corneoscleral button isolation: a surgical procedure to obtain corneoscleral button from porcine eye

corneoscleral-button-isolation-surgical-procedure-to-obtain

Corneoscleral Button Isolation: A Surgical Procedure to Obtain Corneoscleral Button from Porcine Eye

Source: Okurowska, K., et al. Establishing a Porcine Ex Vivo Cornea Model for Studying Drug Treatments against Bacterial Keratitis. J. Vis. Exp. (2020).
...

porcine ex vivo cornea model of bacterial keratitis: a technique to establish bacterial infection in corneal epithelial cells

porcine-ex-vivo-cornea-model-bacterial-keratitis-technique-to

Porcine Ex vivo Cornea Model of Bacterial Keratitis: A Technique to Establish Bacterial Infection in Corneal Epithelial Cells

Source: Okurowska, K., et al. Establishing a Porcine Ex Vivo Cornea Model for Studying Drug Treatments against Bacterial Keratitis. J. Vis. Exp (2020).
...

pig bronchiolar tissue extraction: a procedure to dissect bronchiolar tissue sections from porcine lungs

pig-bronchiolar-tissue-extraction-procedure-to-dissect-bronchiolar

Pig Bronchiolar Tissue Extraction: A Procedure to Dissect Bronchiolar Tissue Sections From Porcine Lungs

Source: Harrington, N. E. et al. Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the ...

ex vivo pig lung model of biofilm: a technique to develop lung model to study bacterial biofilms on pig bronchiolar tissue

ex-vivo-pig-lung-model-biofilm-technique-to-develop-lung-model-to

Ex vivo Pig Lung Model of Biofilm: A Technique to Develop Lung Model to Study Bacterial Biofilms on Pig Bronchiolar Tissue

Source: Harrington, N.E. et al., Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the ...

anterior eye segment dissection: a method to isolate cornea bearing anterior segment from porcine eye

anterior-eye-segment-dissection-method-to-isolate-cornea-bearing

Anterior Eye Segment Dissection: A Method to Isolate Cornea Bearing Anterior Segment from Porcine Eye

Source: Boice, E. N., Snider, E. J. Anterior Segment Organ Culture Platform for Tracking Open Globe Injuries and Therapeutic Performance. J. Vis. Exp. ...

anterior eye segment culturing: a method to culture cornea bearing anterior segment of porcine eye

anterior-eye-segment-culturing-method-to-culture-cornea-bearing

Anterior Eye Segment Culturing: A Method to Culture Cornea Bearing Anterior Segment of Porcine Eye

generation of porcine ventricular slices: a vibratome-based technique to prepare ultra-thin slices of pig heart tissue

generation-porcine-ventricular-slices-vibratome-based-technique-to

Generation of Porcine Ventricular Slices: A Vibratome-based Technique to Prepare Ultra-thin Slices of Pig Heart Tissue

Source: Ou, Q. et. al., Slicing and Culturing Pig Hearts under Physiological Conditions. J. Vis. Exp. (2020)
This video demonstrates a vibratome-based ...

porcine heart excision: a protocol to extract porcine heart for cardiac transplant

porcine-heart-excision-protocol-to-extract-porcine-heart-for-cardiac

Porcine Heart Excision: A Protocol to Extract Porcine Heart for Cardiac Transplant

Source: Hatami, S., et al. Normothermic Ex Situ Heart Perfusion in Working Mode: Assessment of Cardiac Function and Metabolism. J. Vis. Exp. (2019).
In ...

ex situ heart perfusion: a technique to preserve isolated porcine heart function

ex-situ-heart-perfusion-technique-to-preserve-isolated-porcine-heart

Ex Situ Heart Perfusion: A Technique to Preserve Isolated Porcine Heart Function

Source: Hatami, S. et. al., Normothermic Ex Situ Heart Perfusion in Working Mode: Assessment of Cardiac Function and Metabolism. J. Vis. Exp. (2019).
This ...

laser doppler needle probe insertion: a technique for real-time quantification of blood flow within the spinal cord in a porcine model

laser-doppler-needle-probe-insertion-technique-for-real-time

Laser Doppler Needle Probe Insertion: A Technique for Real-time Quantification of Blood Flow within the Spinal Cord in a Porcine Model

Source: Behem, C. R. et al., Real-Time Assessment of Spinal Cord Microperfusion in a Porcine Model of Ischemia/Reperfusion. J. Vis. Exp. (2020).
This ...

intrathecal placement of lumbar drain: a technique to withdraw cerebrospinal fluid from the lumbar spine in porcine model

intrathecal-placement-lumbar-drain-technique-to-withdraw

Intrathecal Placement of Lumbar Drain: A Technique to Withdraw Cerebrospinal Fluid from the Lumbar Spine in Porcine Model

porcine cumulus oocyte complex isolation: a technique to isolate cumulus oocyte complex from porcine ovarian follicle for in vitro fertilization

porcine-cumulus-oocyte-complex-isolation-technique-to-isolate-cumulus

Porcine Cumulus Oocyte Complex Isolation: A Technique to Isolate Cumulus Oocyte Complex from Porcine Ovarian Follicle for In Vitro Fertilization

Source: Gorczyca, G. et al., Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation. J. Vis. Exp. ...

3d culture of porcine cocs: a procedure to encapsulate cumulus oocyte complexes in fibrin-alginate polymer hydrogel beads

3d-culture-porcine-cocs-procedure-to-encapsulate-cumulus-oocyte

3D Culture of Porcine COCs: A Procedure to Encapsulate Cumulus Oocyte Complexes in Fibrin-alginate Polymer Hydrogel Beads

Source: Gorczyca, G., et al. Proteolytically Degraded Alginate Hydrogels and Hydrophobic Microbioreactors for Porcine Oocyte Encapsulation. J. Vis. Exp ...

Encyclopedia Of Experiments - biology