biology

Biology

cancer research

Cancer Research

biological techniques

Biological Techniques

immunology

Immunology

neuroscience

Neuroscience

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microbiology

Microbiology

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jove encyclopedia of experiments

JoVE Encyclopedia of Experiments

Online video encyclopedias of advanced research experiments for scientists in academia and biotech

Research

breast cancer

This collection features biomedical research methods employed in research laboratories to advance breast cancer prevention, detection, and treatment.

Breast Cancer

lung cancer

This collection highlights some of the latest protocols in lung cancer research. The collection covers techniques relating to the generation of lung cancer animal models, cancer cell characterization, treatment and detection strategies, in vitro culture studies, assays and isolation techniques that facilitate lung cancer research.

Lung Cancer

leukemia

Leukemia reflects cancer primarily resulting from an elevated number of white blood cells in the body. This collection features a set of in vitro assay techniques to analyze leukemic cell growth and metabolism, cell isolation and culture methods, approaches to characterize and study cellular morphology, and genetic manipulation procedures.

Leukemia

pancreatic cancer

The collection features protocols demonstrating the generation of pancreatic animal models, genetic engineering of pancreatic cancer cells, methods for in vitro modeling of pancreatic cancer, cell isolation & characterization techniques, and tumor cell behavior and response studies.

Pancreatic Cancer

colorectal cancer

Colorectal cancer originates within the colon or rectal regions of organisms. This collection highlights some fundamental research techniques in colorectal cancer relating to screening tests, genetic analysis, studies on the gut microbiome, therapeutic interventions, animal model generation, assay techniques, and cell behavior & metastatic responses.

Colorectal Cancer

skin cancer

This collection focusses on research approaches relating to melanoma and other types of skin cancer. The protocols feature techniques relevant to developing in vitro and in vivo skin carcinogenesis and metastatic models, cell culturing, skin regeneration approaches, genetic manipulation and sensitivity studies using skin cells, and assay techniques.

Skin Cancer

gynecologic cancer

This collection features protocols relating to the prevention, detection, and treatment of cancer originating from cells or regions of the female reproductive system. The protocols are relevant for cancer-targeting and detection approaches, metabolic assessment of cancer cells, developing in vivo and metastatic animal models, in vitro cell culture and assay techniques, and transplantation experiments.

Gynecologic Cancer

prostate cancer

This collection features techniques employed in prostate cancer research relating to cancer cell/organoid culture, processing, and staining, cell and tissue sampling, organ extractions, in vivo & in vitro cancer modeling, and cancer proteomics studies.

Prostate Cancer

gastrointestinal cancer

Gastrointestinal cancers include various tumor types originating in the regions or organs associated with the gastrointestinal tract. The collection comprises research protocols on cell isolation, culturing, and adhesion studies, tissue staining and histological analysis, DNA methylation studies, tissue preservation methods, exosome analysis, and generation of animal models.

Gastrointestinal Cancer

head and neck cancer

Head and neck cancers constitute carcinomas originating in and around the mucosal surfaces of the head and neck regions. This collection features protocols relating to cancer modeling in vitro, in ovo, and in vivo, cell isolation and culture, fluorescent in situ hybridization-related techniques, generation of decellularized tissue matrices, and drug-resistance characterization assays.

Head and Neck Cancer

urinary tract cancer

This collection features protocols relating to cancers originating in the regions or organs of the urinary or renal system. The techniques include organs, tissue, and cell harvesting procedures, organotypic culturing approaches, in vivo cancer modeling studies, decellularized tissue matrix generation, stem cell-related and electrophysiology studies, genetic-engineering strategies, tissue staining and imaging techniques, and protein expression analysis.

Urinary Tract Cancer

cancers of the nervous system

This collection features protocols relating to cancers originating in the nervous system. The techniques include intra-brain injections, in-vivo and in-vitro tumor modeling, cell and organelle culturing, extracellular vesicle harvesting and purification methodologies, proteomic analysis, organ isolation and preservation methods, cell migration assays, tissue engineering methods, and novel gene delivery approaches.

Cancers of the Nervous System

assays and techniques

Assays and techniques

animal model studies

Animal model studies

preparing organoids from tumors: a 3d in vitro tumor model

preparing-organoids-from-tumors-a-3d-in-vitro-tumor-model

Preparing Organoids from Tumors: A 3D In Vitro Tumor Model

Source: Wadosky, K. M., et al. Generation of Tumor Organoids from Genetically Engineered Mouse Models of Prostate Cancer. J. Vis. Exp. (2019).
Organoids ...

agarose spin down assay: a technique to embed organoids for histological analysis

agarose-spin-down-assay-technique-to-embed-organoids-for-histological

Agarose Spin Down Assay: A Technique to Embed Organoids for Histological Analysis

Source: Lee, S. et al. Establishment and Analysis of Three-Dimensional (3D) Organoids Derived from Patient Prostate Cancer Bone Metastasis Specimens and ...

prostate organoid assay: a matrix gel ring-based ex vivo culture technique to study the differentiation capacity of prostate epithelial cells

prostate-organoid-assay-matrix-gel-ring-based-ex-vivo-culture

Prostate Organoid Assay: A Matrix Gel Ring-based Ex Vivo Culture Technique to Study the Differentiation Capacity of Prostate Epithelial Cells

Source: Crowell, P. D. et al. Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture. J. Vis. Exp. (2019)
In ...

protein lysate extraction: a technique to lyse organoids to collect cellular proteins

protein-lysate-extraction-technique-to-lyse-organoids-to-collect

Protein Lysate Extraction: A Technique to Lyse Organoids to Collect Cellular Proteins

whole-mount immunofluorescence imaging: a fixing and staining technique to evaluate intact organoids

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Whole-mount Immunofluorescence Imaging: A Fixing and Staining Technique to Evaluate Intact Organoids

Source: Crowell, P. D. et al. Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture. J. Vis. Exp. (2019)
This ...

sampling prostate cancer tissue for biobanking: an mri-guided targeted technique to remove biopsy punches from fresh tumor tissue

sampling-prostate-cancer-tissue-for-biobanking-an-mri-guided-targeted

Sampling Prostate Cancer Tissue for Biobanking: An MRI-guided Targeted Technique to Remove Biopsy Punches from Fresh Tumor Tissue

Source: Heavey, S. et al. Use of Magnetic Resonance Imaging and Biopsy Data to Guide Sampling Procedures for Prostate Cancer Biobanking. J. Vis. Exp. ...

functionalized wire-based target cell isolation: an ex vivo technique to isolate cancer cells from spiked peripheral blood

functionalized-wire-based-target-cell-isolation-an-ex-vivo-technique

Functionalized Wire-based Target Cell Isolation: An Ex Vivo Technique to Isolate Cancer Cells from Spiked Peripheral Blood

Source: Chen S. et al. Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization. J. Vis. Exp. (2018)
In this ...

single cell sampling using micromanipulation: a technique to isolate single cells from cancer cell suspension

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Single Cell Sampling Using Micromanipulation: A Technique to Isolate Single Cells from Cancer Cell Suspension

Source: Chen S. et al. Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization. J. Vis. Exp. (2018)
This ...

single cell sampling using laser capture microdissection: a technique to harvest target cells from a heterogeneous cell population

single-cell-sampling-using-laser-capture-microdissection-technique-to

Single Cell Sampling Using Laser Capture Microdissection: A Technique to Harvest Target Cells from a Heterogeneous Cell Population

generating chemoresistant prostate cancer cells: a procedure for obtaining drug-resistant cancer cells in vitro

generating-chemoresistant-prostate-cancer-cells-procedure-for

Generating Chemoresistant Prostate Cancer Cells: A Procedure for Obtaining Drug-resistant Cancer Cells In Vitro

Source: Mohr, L. et al. Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel. J. Vis. Exp. (2017)
This video ...

functional chemoresistance characterization: a method to evaluate drug resistance in cancer cells using clonogenic assay

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Functional Chemoresistance Characterization: A Method to Evaluate Drug Resistance in Cancer Cells Using Clonogenic Assay

adi-based autophagy induction in prostate cancer cells: an enzyme-based technique to measure autophagic response in cells

adi-based-autophagy-induction-prostate-cancer-cells-an-enzyme-based

ADI-based Autophagy Induction in Prostate Cancer Cells: An Enzyme-based Technique to Measure Autophagic Response in Cells

Source: Changou, C. A. et al. Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy. J. Vis. Exp. (2013)
In this video, we ...

protein extraction and proteolysis: a method to obtain proteins from prostate tumor tissue samples and their enzymatic digestion into peptides

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Protein Extraction and Proteolysis: A Method to Obtain Proteins from Prostate Tumor Tissue Samples and their Enzymatic Digestion into Peptides

Source: Cheng, L. C. et al. Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in ...

peptide purification: an rp-hplc-based technique to extract peptides from digested protein lysates

peptide-purification-an-rp-hplc-based-technique-to-extract-peptides

Peptide Purification: An RP-HPLC-based Technique to Extract Peptides from Digested Protein Lysates

phosphopeptide enrichment: an antibody-based immunoprecipitation technique to separate specific phosphorylated peptides from complex peptide mixtures

phosphopeptide-enrichment-an-antibody-based-immunoprecipitation

Phosphopeptide Enrichment: An Antibody-based Immunoprecipitation Technique to Separate Specific Phosphorylated Peptides from Complex Peptide Mixtures

Source: Cheng, L. C., et al. Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in ...

titanium dioxide-based enrichment of phosphopeptides: a method for the selective isolation of phosphopeptides from peptide library

titanium-dioxide-based-enrichment-phosphopeptides-method-for

Titanium Dioxide-based Enrichment of Phosphopeptides: A Method for the Selective Isolation of Phosphopeptides from Peptide Library

extracting prostate from mouse urogenital system: a technique to separate prostate gland from other urinary and genital system organs in male mouse

extracting-prostate-from-mouse-urogenital-system-technique-to

Extracting Prostate from Mouse Urogenital System: A Technique to Separate Prostate Gland from Other Urinary and Genital System Organs in Male Mouse

Source: Nath, D. et al. Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models. J. ...

individual lobe extraction from prostate: a technique to harvest individual prostate lobes from mouse prostate

individual-lobe-extraction-from-prostate-technique-to-harvest

Individual Lobe Extraction from Prostate: A Technique to Harvest Individual Prostate Lobes from Mouse Prostate

intra-prostatic injection of cancer cells: a technique to deliver cancer cells for establishing orthotopic prostate cancer mouse model

intra-prostatic-injection-cancer-cells-technique-to-deliver-cancer

Intra-prostatic Injection of Cancer Cells: A Technique to Deliver Cancer Cells for Establishing Orthotopic Prostate Cancer Mouse Model

Source: Anker, J. F. et al. Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate ...

renal capsule tumor xenografting: a technique to generate an experimental animal model to evaluate prostate carcinogenesis

renal-capsule-tumor-xenografting-technique-to-generate-an

Renal Capsule Tumor Xenografting: A Technique to Generate an Experimental Animal Model to Evaluate Prostate Carcinogenesis

Source: Nicholson, T. M. et al. Renal Capsule Xenografting and Subcutaneous Pellet Implantation for the Evaluation of Prostate Carcinogenesis and Benign ...

modeling prostate cancer in genetically-engineered mouse models: a crispr/cas9-mediated localized gene editing technique in mouse anterior prostate lobe cells

modeling-prostate-cancer-genetically-engineered-mouse-models

Modeling Prostate Cancer in Genetically-engineered Mouse Models: A CRISPR/Cas9-mediated Localized Gene Editing Technique in Mouse Anterior Prostate Lobe Cells

Source: Riedel, M. et al. Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration. J. Vis. Exp. (2018)
This video describes a method ...