Showing 0 results for "Transformation Of UAS cDNA ORF Plasmids From CRISPR Based Modular Assembly Into E Coli 1 Pre chill 10 L Tips And 1 5 ML Tubes In An Aluminum Cooling Block On Ice 2 Thaw Competent E Coli Cells On Ice For 5 Minutes 3 Add 10 L Of Competent Cells To Each Pre chilled 1 5 ML Tube 4 Add 1 L Of The UAS CDNA ORF Plasmid Products To Each Tube 5 Gently Swirl The Tubes To Mix And Incubate On Ice For 30 Minutes 6 Heat Shock The Cells At 42 C For 1 Minute 7 Immediately Chill The Tubes On Ice For 2 Minutes 8 Add 100 L Of Pre warmed 37 C SOC Medium To Each Tube 9 Incubate The Tubes In A Shaking Incubator At 250 RPM And 37 C For 1 Hour 10 Warm The LB Plates Containing The Appropriate Antibiotic To 37 C 11 Spread The Cells Onto The Warm Plates And Incubate At 37 C Overnight"