This protocol is an excerpt from Parker&#160;et al., Studying Mitochondrial Structure and Function in Drosophila Ovaries,&#160;J. Vis. Exp. (2017).
1. Preparation of Drosophila (the tools required are depicted in Figure 1A)
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	<li>For any of the experiments described, collect Drosophila (maintained at room temperature, or 25 &#176;C) within 5 days of eclosion and place them in a vial filled with 5 ...

drosophila melanogaster ovary dissection: a technique for ex vivo tissue preparation

Source: Parker, D. J., et al. <a href="https://www.jove.com/video/54989/" target="_blank">Studying Mitochondrial Structure and Function in Drosophila Ovaries</a>. J. Vis. Exp. (2017).
This video describes the general anatomy of the Drosophila&#160;ovary and features&#160;a method of dissecting ovaries for live or fixed imaging.

<img alt="Figure 1" src="/files/ftp_upload/20101/20101fig1.jpg" /> 
Figure 1: Experimental Plan and Preparation. (A) Tools used in the methods described: A. Fly vial; B. Insect dissecting medium; C. Paraformaldehyde; D. Fly brush; E. Dissecting dish; F. Cover glass; G. Microfuge tube. H. Glass-bottomed dish; I. Glass slide; J. 1,000-&#181;L micropipette; K. 200-&#181;L micropipette; L. 2.5-&#181;L micropipette; M. Teasing needle with a bent tip (tip ...

Drosophila melanogaster Ovary Dissection: A Technique for Ex Vivo Tissue Preparation

Source: Parker, D. J., et al. Studying Mitochondrial Structure and Function in Drosophila Ovaries. J. Vis. Exp. (2017).
This video describes the general ...

- In order to isolate ovaries from live Drosophila flies, begin by moving an anesthetized female to one of three dissection wells filled with pre-warmed insect dissection medium. The medium will help maintain tissue integrity and viability.
Under a dissection microscope, separate the abdomen from the thorax. Then, move the abdomen into a new well. To isolate the ovaries, hold the abdomen at the posterior end and carefully scrape out the reproductive organs. Lastly, transfer the ovaries into a fresh well. After this step, you may either use the whole ovary or further dissect it into its component structures.
Each Drosophila ovary is composed of several ovarioles, consisting of a chain of egg chambers with increasing levels of maturity. To free the ovarioles, pin down the ovary at the opaque posterior end and carefully tease the protective sheath from around the ovary until ovarioles are properly separated.
In the example protocol, we will show the dissection of Drosophila ovaries for their use in live and fixed tissue microscopy.
- After sorting out five anesthetized female flies according to the text protocol, under the dissection microscope, use two pairs of forceps to sever the thorax from the abdomen. Then, carefully transfer the abdomens to the second well of the dish. Using one pair of forceps to hold the abdomen at the posterior end, use the other pair of forceps to slowly push the ovaries out.
Then, use the forceps to hold an individual ovary by the opaque posterior end and carefully move it to the third well of the dish for teasing or fixing. Carefully tease the protective sheath from around the ovaries by using a pair of forceps to hold the posterior end and lightly sweeping a teasing needle from the posterior to the anterior end.

drosophila-melanogaster-ovary-dissection-technique-for-ex-vivo-tissue

Research

JoVE Encyclopedia of Experiments

Biology

Drosophila melanogaster (fruit fly)

Adult

This video describes the general anatomy of the Drosophila&#160;ovary and features&#160;a method of dissecting ovaries for live or fixed imaging.

Overview

Protocol

<img title="figure-representative results-58" alt="figure-representative results-58" src="/files/ftp_upload/20101/20101fig1.jpg" /> 
Figure 1: Experimental Plan and Preparation. (A) Tools used in the methods described: A. Fly vial; B. Insect dissecting medium; C. Paraformaldehyde; D. Fly brush; E. Dissecting dish; F. Cover glass; G. Microfuge tube. H. Glass-bottomed dish; I. Glass slide; J. 1,000-&#181;L micropipette; K. 200-&#181;L micropipette; L. 2.5-&#181;L micropipette; M. Teasing needle with a bent tip (tip ...

Drosophila melanogaster Ovary Dissection: A Technique for Ex Vivo Tissue Preparation

In This Article

Overview

Protocol

Representative Results