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In This Article

  • Overview
  • Protocol
  • Representative Results
  • Reprints and Permissions

Overview

Drosophila fruit flies can distinguish small changes in temperature and therefore select environments with favorable thermal conditions. This video describes a behavioral assay that tests the temperature preference of Drosophila larvae on a linear thermal gradient.

Protocol

This protocol is an excerpt from Liu et al. A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae, J. Vis. Exp. (2018).

1. Temperature Gradient Setup

  1. Prepare single-directional gradient
    1. To create a humid ambient environment during the assays, place the two aluminum blocks connected to two water baths on wet paper towels, separated by 10 cm (Figure 1A).

Representative Results

figure-representative results-25
Figure 1: Single and bidirectional gradient assay setups. (A) Single-directional gradient setup with two aluminum assay plates on two aluminum blocks. The temperatures of the aluminum blocks are controlled by circulating water from two water baths. (B) The arrangement of the three aluminum blocks, water baths and an aluminum plate (250 x 220 mm) for a bidirectional gradient. The left.......

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