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Drosophila Activity Monitor (DAM): A Method to Measure Locomotor Activity in Flies
Overview
This video describes the Drosophila activity monitor (DAM) system used to track locomotor activity. Researchers use activity data collected from the DAM to study circadian rhythms in fruit flies. The featured protocol clip shows how to load flies in the device and record activity data for circadian experiments.
Protocol
This protocol is an excerpt from Chiu et al., Assaying Locomotor Activity to Study Circadian Rhythms and Sleep Parameters in Drosophila, J. Vis. Exp. (2010).
1. Loading Flies into Activity Tubes and Locomotor Activity Monitoring System
- Prior to loading flies into activity tubes, turn on the incubators that will be used to house the activity monitors. Adjust the temperature using the incubator controls and set the light/dark r.......
Representative Results
Figure 1: Eduction graphs generated using FaasX showing daily locomotor activity rhythms of rhythmic wild type flies (w per0 flies carrying a per+ transgene) (A and B) vs. arrhythmic w per0 mutants (C and D). Male flies were kept at 25 °C and entrained for 4 days in 12:12 LD (light: dark) cycles followed by seven days in DD (constant darkness). For each fly line, the locom.......
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Source: Chiu, J. C., et al. Assaying Locomotor Activity to Study Circadian Rhythms and Sleep Parameters in Drosophila. J. Vis. Exp. (2010).
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