Zebrafish Xenograft Assay: A Method to Assess the Anti-cancer Efficacy of Therapeutics on Tumorigenicity in Zebrafish In Vivo

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Zebrafish Xenograft Assay

NOTE: This technical approach is visualized as a schematic (Figure 1).

1. Preparation of stock reagents
   1. Prepare 1 L of 30x Danieau’s solution: 1740 mM NaCl, 21 mM KCl, 12 mM MgSO₄•7H2O, 18 mM Ca(NO