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Individual Lobe Extraction from Prostate: A Technique to Harvest Individual Prostate Lobes from Mouse Prostate
Overview
This video describes a technique to harvest individual lobes from the prostate gland of a mouse model. Individual lobes can then be used for histological analysis and cancer research.
Protocol
All procedures involving animals have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Gross Anatomy of the Prostate and Individual Lobe Microdissection
NOTE: This is depicted in Figures 1(i-n) and Figure 2
- Flip the tissue with a pair of blunt forceps so that the dorsal side faces up, showing the dorsal lobes, which resemble a butte.......
Representative Results
Figure 1: Dissection of the mouse prostate. Step-by-step images for the dissection of the mouse prostate from the UGS: (a) removing fat, (b) removing the urinary bladder, (c) removing the vas deferens, (d) ventral view of the prostate with the urethra, (e) dorsal view of the prostate with the urethra, (f-.......
This article has been published
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Source: Nath, D. et al. Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models. J. Vis. Exp. (2018)
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