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Whole Mount Preparation of Mouse Intestine: Using a Simple Gut-incising Device to Prepare Whole Mount
Overview
In this video, we demonstrate whole mount preparation of the mouse intestine using a simple gut-incising device. The resulting mounts are of superior and uniform quality and can be used for histological analyses.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Device
- Machine the main portion of the base of the device from out of a solid block of high-density acetyl resin, as described in this manuscript. At each end, create 3 mm high-elevated strips and part machine out of these 2.5 mm grooves to take the rods (see Figure 1 and .......
Representative Results
Figure 1. Photos of the original device. The original device was constructed from several pieces of metal, prepared by hand. (A) The device assembled. (B) The top of the device has been removed to show the rods in position.
This article has been published
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Source: Yoneda, M. et al. A Simple Device to Rapidly Prepare Whole Mounts of the Mouse Intestine. J. Vis. Exp. (2015)
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