eoe sub articles

EoE Sub Articles

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. UCC Mouse Model
<ol>
	<li>Preparation of mice for procedure
	<ol>
		<li>Obtain 6- to 8-week old female NOD/SCID mouse. Shave the lower backs of the mouse using hair removal cream. Anesthetize the mouse in an induction chamber with isoflurane (2.5% in 100% oxygen, 1 L/min).</li>
		<li>Once sedated, place the mouse in the supine po...

patient-derived tumor xenografting: a technique to generate experimental mouse model for evaluating urothelial cell carcinoma

Source: Moret, R. et al. <a href="https://www.jove.com/video/59223" target="_blank">Patient-derived Orthotopic Xenograft Models for Human Urothelial Cell Carcinoma and Colorectal Cancer Tumor Growth and Spontaneous Metastasis.</a> J. Vis. Exp. (2019)
This video describes the procedure to generate an experimental xenograft murine model, which can be used for studying urothelial cell carcinoma progression.

<img alt="Figure 1" src="/files/ftp_upload/20551/20551_Figure1.jpg" /> 
Figure 1: Orthotopic UCC mouse models. (Aa) An angiocatheter was inserted into the bladder of a female NOD/SCID mouse and an electrocautery shock was applied to the bladder wall via a guidewire. (Ab) Luciferase tagged UCC tumor cells, BlCaPt15 (2 x 104&#160;cells), or BlCaPt37 (5 x 105&#160;cells) with the addition of 3 x 105&#160;</sup...

Patient-derived Tumor Xenografting: A Technique to Generate Experimental Mouse Model for Evaluating Urothelial Cell Carcinoma

Source: Moret, R. et al. Patient-derived Orthotopic Xenograft Models for Human Urothelial Cell Carcinoma and Colorectal Cancer Tumor Growth and ...

For a patient-derived urothelial cell carcinoma or UCC mouse model generation, an immunocompromised mouse is inoculated with human UCC cells to study cancer progression and metastasis.
To begin xenografting, take an immunocompromised anesthetized female mouse. Shave the lower back of the mouse to expose its pelvic region. Now, place the mouse in the supine position with its exposed skin in contact with a dispersive pad that serves as a grounding electrode to complete the circuit during electrical stimulation.
Next, take an angiocatheter, a thin hollow tube, attached to a guidewire. Lubricate the surface of the catheter and insert it into the mouse&#39;s urethra to access its bladder. Place an active electrode on the guidewire to stimulate the bladder wall. This procedure helps rupture the mucopolysaccharide layer, a protective barrier of the bladder mucosa, and aids in the subsequent engraftment of tumor cells.
Now, gently remove the angiocatheter-guidewire assembly from the urethra. Take a second angiocatheter attached to a syringe containing the UCC cell suspension and insert it into the mouse&#39;s urethra. Inject the UCC cells into the mouse&#39;s bladder. Cancer cells interact with the bladder mucosa to form a tumor that may eventually spread to other tissues and organs.

Patient-derived Tumor Xenografting: A Technique to Generate Experimental Mouse Model for Evaluating Urothelial Cell Carcinoma

In This Article

Overview