A subscription to JoVE is required to view this content. Sign in or start your free trial.
Focus Ultrasound Based Microbubble Mediated Blood-Brain Barrier Opening: A Technique to Create Localized Transient Openings in Blood-Brain Barrier of Mouse by Sonoporation
In This Article
Overview
This video describes a method of temporarily disrupting the blood-brain barrier (BBB) by creating transient openings using microbubble-mediated focused ultrasound (FUS). These transient local BBB openings can eventually be used to deliver larger-sized drug molecules directly into the brain parenchyma.
Protocol
All procedures involving human participants have been performed in compliance with the institutional, national and international guidelines for human welfare and have been reviewed by the local institutional review board.
1. Focused Ultrasound System
NOTE: The described setup is an in-house built BBB disruption system based on commercially available components and includes a 3D-printed custom-made cone and detachable stereotactic platform. The system is designed modular, which facilitates modifications according to available equipment and specific use. The p....
Representative Results
Figure 1: Focused ultrasound setup. (A) Schematic representation of the focused ultrasound setup. (B) Picture of the focused ultrasound setup. The system consists of a top-down mounted transducer on a 1D linear stage over a second 2D stage for automatic 3D positioning. The transducer is built in a water-filled beam-cone, closed at the bottom with an acoustically .......
This article has been published
Video Coming Soon
Source: Haumann, R. et al. A High-Throughput Image-Guided Stereotactic Neuronavigation and Focused Ultrasound System for Blood-Brain Barrier Opening in Rodents. J. Vis. Exp. (2020)
ABOUT JoVE
Copyright © 2025 MyJoVE Corporation. All rights reserved