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Cerebellum Dissection and Slice Preparation: A Method to Generate Tissue Slices from Mouse Cerebellum
Overview
This video describes a method to generate tissue slices from the mouse cerebellum. The cerebellar slices can be used for organotypic culturing that provides insights into neurodevelopmental mechanisms, including myelination dynamics.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Cerebellum Dissection and Sections Preparation (Hands-on Time ≈ 15–20 min per animal)
- Place one of the 60 mm cell culture dishes containing the ice-cold dissection medium under the binocular microscope and remove the top plate.
- Swab the head fur with 70% ethanol (carefully avoiding .......
Representative Results
Figure 1: Illustration of cerebellum slices generation. The dissection is divided in three main steps. (A) The cerebellum is isolated, and the meninges removed (steps 1.10 and 1.11). (B) The cerebellum is then transferred to the chopper platform and sliced (steps 1.12 to 1.16). (C) Lastly, the slices are isolated from the vermis .......
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Source: Thetiot, M. et al. Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures. J. Vis. Exp. (2019)
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