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Murine Model of Islet Transplantation: A Surgical Method to Infuse Pancreatic Islets in Portal Vein and Prevention of Postoperative Bleeding
In This Article
Overview
In this video, we describe a procedure to infuse pancreatic islet cells into the hepatic portal vein, followed by application of gel foam to successfully prevent bleeding. Postoperative bleeding is a common cause of high mortality during islet transplantation.
Protocol
1. Islet transplantation
- Induce and maintain the mouse under general anesthesia with 2% isoflurane. Check for the lack of pedal reflexes to ensure proper anesthetization of the animal.
- Shave and remove the fur in the abdomen area of the mouse.
- Administer a single pre-operative dose of Buprenorphine (0.1 mg/kg i.p.).
- Disinfect the surgical area with three alternating wipes of 2% iodine and 75% alcohol.
- Perform a laparotomy with micro scissors to generate a 1-1.5 cm incision.
- Open the peritoneal cavity with a retractor. Follow with either method A or method B as detailed below.
Representative Results
Figure 1: Illustration of intrahepatic islet transplantation procedures. (A, C). Schematics of key steps used in Method A and Method B. (B, D). Islets were injected directly via the portal vein (C) or indirectly via fat pat (D).
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Source: Gou, W. et al. Minimizing Post-Infusion Portal Vein Bleeding during Intrahepatic Islet Transplantation in Mice. J. Vis. Exp. (2021)
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