eoe sub articles

EoE Sub Articles

1. Islet transplantation
<ol>
	<li>Induce and maintain the mouse under general anesthesia with 2% isoflurane. Check for the lack of pedal reflexes to ensure proper anesthetization of the animal.</li>
	<li>Shave and remove the fur in the abdomen area of the mouse.</li>
	<li>Administer a single pre-operative dose of Buprenorphine (0.1 mg/kg i.p.).</li>
	<li>Disinfect the surgical area with three alternating wipes of 2% iodine and 75% alcohol.</li>
	<li>Perform a laparotomy with micro scissors to generate...

murine model of islet transplantation: a surgical method to infuse pancreatic islets in portal vein and prevention of postoperative bleeding

Source: Gou, W. et al. <a href="https://www.jove.com/v/62530">Minimizing Post-Infusion Portal Vein Bleeding during Intrahepatic Islet Transplantation in Mice.</a> J. Vis. Exp. (2021)
In this video, we describe a procedure to infuse pancreatic islet cells into the&#160;hepatic portal vein, followed by application of gel foam to successfully prevent bleeding. Postoperative bleeding is a common cause of high mortality during islet transplantation.

<img alt="Figure 1" src="/files/ftp_upload/20790/20790_Figure1.jpg" /> 
Figure 1: Illustration of intrahepatic islet transplantation procedures.&#160;(A,&#160;C). Schematics of key steps used in Method A and Method B. (B,&#160;D). Islets were injected directly via the portal vein (C) or indirectly via fat pat (D).

Murine Model of Islet Transplantation: A Surgical Method to Infuse Pancreatic Islets in Portal Vein and Prevention of Postoperative Bleeding

Source: Gou, W. et al. Minimizing Post-Infusion Portal Vein Bleeding during Intrahepatic Islet Transplantation in Mice. J. Vis. Exp. (2021)
In this video, ...

In a mouse model of insulin-dependent diabetes, pancreatic islets are injected via the hepatic portal vein for therapeutic purposes. The beta cells within the islet cluster produce insulin, a hormone that regulates blood glucose levels.
Intraportal injection is a simple and effective technique, but postoperative bleeding often leads to the death of the model animal. To prevent this complication, prep an anesthetized mouse in the supine position. Shave the abdominal area and apply a disinfectant solution. Make an incision to expose the peritoneal cavity. Exteriorize the intestines from the peritoneal cavity on a moist gauze to expose the underneath portal vein.
Take an insulin syringe pre-loaded with islets. Insert the needle directly parallel into the portal vein, with its bevel facing down. Draw some blood into a syringe to mix the islets, and then inject slowly. Pull and push the plunger multiple times to infuse the entire islet suspension. Now, place a gelatin sponge to cover the injection site.
Gelatin, a hemostatic matrix, absorbs blood and swells to form a stable clot over the puncture site, thereby arresting the bleeding. Next, return the intestine into the peritoneal cavity and suture the incision. Return the mouse to its home cage and regularly monitor for signs of recovery.

murine-model-islet-transplantation-surgical-method-to-infuse

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In this video, we describe a procedure to infuse pancreatic islet cells into the&#160;hepatic portal vein, followed by application of gel foam to successfully prevent bleeding. Postoperative bleeding is a common cause of high mortality during islet transplantation.

Overview

Protocol

<img title="figure-representative results-58" alt="figure-representative results-58" src="/files/ftp_upload/20790/20790_Figure1.jpg" /> 
Figure 1: Illustration of intrahepatic islet transplantation procedures.&#160;(A,&#160;C). Schematics of key steps used in Method A and Method B. (B,&#160;D). Islets were injected directly via the portal vein (C) or indirectly via fat pat (D).

Murine Model of Islet Transplantation: A Surgical Method to Infuse Pancreatic Islets in Portal Vein and Prevention of Postoperative Bleeding

In This Article

Overview

Protocol

Representative Results

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