Harvesting Articular Cartilage From Joints of an Equine Donor: A Surgical Procedure to Isolate Vascular Connective Tissue From Cadaveric Joints of an Equine Donor

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board

1. Harvesting of Articular Cartilage from Donor (Cadaveric) Joints

1. Ahead of the harvesting step, prepare 1 L of cartilage washing solution, consisting of sterile phosphate-buffered saline (PBS), supplemented with 100 U/mL penicillin, 100 µg/mL streptomycin, and 1% (v/v) Amphotericin B.
2. Wear gloves and a lab coat during the entire harvesting procedure, since the donor can carry pathogens.
3. Obtain an intact (cadaveric) joint for cartilage isolation.
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