eoe sub articles

EoE Sub Articles

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Cannulation of the Mesenteric Lymph Duct
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	<li>Place the animal with its right side facing toward the operator. Perform the surgery with or without the assistance of a surgical microscope as required.</li>
	<li>Open the top layer of the abdominal muscle wall with a straight 4 cm incision extending from the midline (xiphoid pr...

mesenteric lymph duct cannulation in a rat model to harvest lymph fluid

Source: Trevaskis, N. L., et al. <a href="https://app.jove.com/v/52389">The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport.</a> J. Vis. Exp. (2015).
This video demonstrates a technique to cannulate the mesenteric lymph duct in rats. This method enables direct access to the entire volume of components present in the lymph fluid.

<img alt="Figure 1" src="/files/ftp_upload/21759/21759_Figure1.jpg" /> 
Figure 1. Photographs of (A) the abdomen shaved in preparation to cannulate the lymph duct, (B) the abdominal muscle wall opened with a straight 4 cm incision extending from the midline to the right flank to access the lymph duct, and (C) the superior mesenteric lymph duct (in yellow circle) perpendicular to the right kidney.

Mesenteric Lymph Duct Cannulation in a Rat Model to Harvest Lymph Fluid

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. ...

Begin with a surgically intervened rat with its abdomen exposed.&#160;
Locate the superior mesenteric lymph duct, lying perpendicular to the right kidney and positioned in front and parallel to the mesenteric artery.
Pass straight forceps through the peri-renal fat bed.
Pull a lymph cannula through the peri-renal fat bed, positioning one end adjacent to the lymph duct and allowing the other end to protrude outside the animal&#39;s body.
Next, create a small incision in the lymph duct.
Fill the cannula with an anticoagulant solution and insert it into the lymph duct.
Inside the duct, the lymph fluid contains several biomolecules including clotting factors that trigger a cascade of events leading to clot formation.
However, the anticoagulant binds to the clotting factors, inhibiting their activity.&#160;&#160;
This enables unclotted lymph fluid to flow freely through the cannula.
Apply a veterinary adhesive over the entrance hole to secure the cannula in place.