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In This Article

  • Overview
  • Protocol
  • Results
  • Disclosures
  • Materials

Overview

This video demonstrates a technique to cannulate the mesenteric lymph duct in rats. This method enables direct access to the entire volume of components present in the lymph fluid.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Cannulation of the Mesenteric Lymph Duct

  1. Place the animal with its right side facing toward the operator. Perform the surgery with or without the assistance of a surgical microscope as required.
  2. Open the top layer of the abdominal muscle wall with a straight 4 cm incision extending from the midline (xiphoid process) to the right flank approximately 2 cm below the ribcage (costal margin) using a sterile scalpel blade (see Figure 1B).
  3. <....

Results

figure-results-58
Figure 1. Photographs of (A) the abdomen shaved in preparation to cannulate the lymph duct, (B) the abdominal muscle wall opened with a straight 4 cm incision extending from the midline to the right flank to access the lymph duct, and (C) the superior mesenteric lymph duct (in yellow circle) perpendicular to the right kidney.

....

Disclosures

No conflicts of interest declared.

Materials

NameCompanyCatalog NumberComments
Sterile salineBaxter healthcareAHB 1307Any brand can be used. Example here is Baxter 100 ml saline bags, box of 50
70 % ethanol in waterAnyAny brand can be used
Chlorhexidine gluconate solution (Microshield 4)Livingstone InternationalJJ60243LAny brand can be used. http:// www.livingstone.com.au/? PG=search_result&CAT=6&search =JJ60243L
Betadine solutionLivingstone InternationalBU0510Any brand can be used. http:// www.livingstone.com.au/? PG=search_result&CAT=6&search =BU0510
Ilium Ketamil (Ketamine 100 mg/ ml)PROVET VICTORIAKETA I 1http://www.provet.vom.au
Heparin (35000I.U. in 35 mLSigmaPharmaceuticals337220http://www.sigmaco.com.au
Polyethylene (PE) cannula o.d. 0.96 mm x i.d. 0.58 mmMicrotube extensionsPE8050Any brand can be used. Example here is PE tubing 0.96 x0.58 mm, 30 m
Polyethylene (PE) cannula o.d. 0.8
mm x i.d. 0.5 mm
Microtube extensionsPE9658Any brand can be used. Example here is PE tubing 0.8 x0.5 mm, 30 m
MarkersAnyAny brand can be used
Veterinary adhesiveAnyAny brand can be used
23 gauge needlesLivingstone InternationalDN23GX0.75LVAny brand can be used. Example here is Livingstone Disposable Needle, Sterile, 23GX0.75inch, 100/BOX. http:// www.livingstone.com.au/? PG=search_result&CAT= 6&search=DN23GX0.75LV
25 gauge needlesLivingstone InternationalDN25GX1.0LVAny brand can be used. Example here is Livingstone Disposable Needle, Sterile, 25GX1.0inch, 100/BOX. http:// www.livingstone.com.au/? PG=search_result&CAT=6&search= DN25GX1.0LV
1 ml syringeLivingstone InternationalT3SS01TAAny brand can be used. Example here is Terumo syringe 1 ml Slip Tuberculin 100/Box. http:// www.livingstone.com.au/? PG=search_result&CAT=6&search =T3SS01TA
10 ml syringeLivingstone InternationalT3SS10SAAny brand can be used. Example here is Terumo syringe 10 ml Slip 100/Box. http:// www.livingstone.com.au/? PG=search_result&CAT=6&search =T3SS10SA
Gauze swabsLivingstone InternationalGSC075Any brand can be used and cut to required size. Example here is gauze swabs cotton filled 7.5x7.5 cm, 8 ply. http:// www.livingstone.com.au/? PG=search_result&CAT=6&search =GSC075
Heating padRatekWT1Any brand that keeps temperature at 37C can be used. Example here is Ratek warming tray.
Surgical microscopeZeiss495005-0014-000Any brand can be used. Example here is Zeiss Stereomicroscope Stemi 2000-C with Stand S Double Spot and KL 300 LED. https:// www.micro-shop.zeiss.com/? l=en&p=us&f=e&i=10143
1 x Small surgical scissorsFine Science Tools (FST)14060-09Any brand can be used. Example here is FST Fine Scissors, 9 cm with 21 mm cutting edge, sharp, straight. http://www.finescience.ca/ Special-Pages/Products.aspx? ProductId=40&CategoryId=17
2 x Forceps with serrated curved tipFine Science Tools (FST)11001-13Any brand can be used. Example here is FST 13 cm standard pattern forceps with curved 2.8x1.4 mm tip. http://www.finescience.ca/ Special-Pages/Products.aspx? ProductId=405&CategoryId=32

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