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Encyclopedia of Experiments

Cannulation of the Carotid Artery in a Rat Model

In this video, we demonstrate a surgical procedure to cannulate the left carotid artery in a rat model.

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Cannulation of the Carotid Artery

The carotid artery cannulation may be performed before or after the cannulation of the mesenteric lymph duct. Some operators prefer to cannulate the carotid artery prior to cannulating the lymph duct so as to reduce potentially dislodging the lymph cannula when moving the .......

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Figure 1
Figure 1. Photographs of (A) the carotid artery isolated with two silk sutures with the one closest to the operator tied off, (B) the carotid artery isolated and blood flow occluded with a pair of straight fine tip forceps placed underneath the artery, and (C) a carotid artery with the cannula in place and the patency being checked by drawing bac.......

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