eoe sub articles

EoE Sub Articles

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Cannulation of the Carotid Artery
The carotid artery cannulation may be performed before or after the cannulation of the mesenteric lymph duct. Some operators prefer to cannulate the carotid artery prior to cannulating the lymph duct so as to reduce potentially dislodging the lymph cannula when moving the ...

cannulation of the carotid artery in a rat model

Source: Trevaskis, N. L. et al., <a href="https://app.jove.com/v/52389">The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport.</a> J. Vis. Exp. (2015)
In this video, we demonstrate a surgical procedure to cannulate the left carotid artery in a rat model.

<img alt="Figure 1" src="/files/ftp_upload/21761/21761_Figure1.jpg" /> 
Figure 1. Photographs of (A) the carotid artery isolated with two silk sutures with the one closest to the operator tied off, (B) the carotid artery isolated and blood flow occluded with a pair of straight fine tip forceps placed underneath the artery, and (C) a carotid artery with the cannula in place and the patency being checked by drawing bac...

Cannulation of the Carotid Artery in a Rat Model

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. ...

Prepare an anesthetized rat in a supine position.
Incise longitudinally through the skin above the anatomical left aspect of the trachea.
Dissect the subcutaneous connective tissue. Retract the neck muscles, revealing the left carotid artery.
Isolate an artery section and place silk sutures around both ends.
Ligate the suture placed distal from the heart to prevent blood flow toward the head region.
Obstruct blood flow through the artery section. Make an incision on the top surface of the artery.
Insert an anticoagulant-filled cannula connected to an anticoagulant-filled syringe into the incised section.
Advancing the cannula to the predetermined depth, inject the anticoagulant solution into the cannula.
Draw back a small blood volume through the cannula to confirm unobstructed flow.
Flush with the anticoagulant solution and seal the cannula&#39;s end to prevent blood leakage.
Secure the cannula within the artery. Close the surgical incision.
The cannulated rat model is ready for experiments.