Establishing a Mouse Model of Pleural Disseminated Lung Cancer

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Generation of a pleural dissemination model

1. Prepare luciferase-expressing target cells and suspend 1.0 × 10⁶ target cells in 100 µL of phosphate-buffered saline (PBS)
   NOTE: Intrathoracic cancer cells such as lung cancer and MPM are suitable as target cells. Luciferase-expressing cells were prepared via luciferase gene transfection, and high expression of luciferase was confirmed after > 10 cell passages. Cells....

Results

Figure 1: Easy hand-made device for cell transplantation. Attach the stopper made with polystyrene foam to the 30G needle so that the tip remains at 5 mm. The tip of the needle should be bent to avoid pneumothorax.

Figure 2: Injection of target cells in.......

Materials

Name	Company	Catalog Number	Comments
1mL syringe	TERUMO	SS-01T	for mice experiment
30G needle	Nipro	1907613	for mice experiment
BALB/cSlc-nu/nu	Japan SLC
D-Luciferin (potassium salt)	Cayman Chemical	14681	for bioluminescence imaging and DLIT
Isoflurane	Wako	095-06573	for mice anesthesia
0.25w/v% Trypsin-1mmol/l EDTA 4Na Solution with Phenol Red	Wako	209-016941	for cell culture
RPMI-1640 with L-glutamine and Phenol Red	Wako	189-02025	for cell culture