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Establishing a Mouse Model of Pleural Disseminated Lung Cancer

This video demonstrates a method to generate a mouse model of pleural dissemination by injecting lung cancer cell suspension into the thoracic cavity of an immunodeficient mouse.

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Generation of a pleural dissemination model

  1. Prepare luciferase-expressing target cells and suspend 1.0 × 106 target cells in 100 µL of phosphate-buffered saline (PBS)
    NOTE: Intrathoracic cancer cells such as lung cancer and MPM are suitable as target cells. Luciferase-express.......

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Figure 1
Figure 1: Easy hand-made device for cell transplantation. Attach the stopper made with polystyrene foam to the 30G needle so that the tip remains at 5 mm. The tip of the needle should be bent to avoid pneumothorax.

Figure 2
Figure 2: Injection of target cells in.......

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