eoe sub articles

EoE Sub Articles

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Generation of a pleural dissemination model
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	<li>Prepare luciferase-expressing target cells and suspend 1.0 &#215; 106 target cells in 100 &#181;L of phosphate-buffered saline (PBS) 
	NOTE: Intrathoracic cancer cells such as lung cancer and MPM are suitable as target cells. Luciferase-express...

establishing a mouse model of pleural disseminated lung cancer

Source: Yasui, H., et al. <a href="https://app.jove.com/v/61593">Near Infrared Photoimmunotherapy for Mouse Models of Pleural Dissemination.</a> J. Vis. Exp. (2021)
This video demonstrates a method to generate a mouse model of pleural dissemination by injecting lung cancer cell suspension into the thoracic cavity of an immunodeficient mouse.

<img alt="Figure 1" src="/files/ftp_upload/21762/21762_Figure1.jpg" /> 
Figure 1: Easy hand-made device for cell transplantation. Attach the stopper made with polystyrene foam to the 30G needle so that the tip remains at 5 mm. The tip of the needle should be bent to avoid pneumothorax.
<img alt="Figure 2" src="/files/ftp_upload/21762/21762_Figure2.jpg" /> 
Figure 2: Injection of target cells in...

Establishing a Mouse Model of Pleural Disseminated Lung Cancer

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. ...

To begin, prepare an immunodeficient mouse.
Using an assembly with a bent needle tip, inject a lung cancer cell suspension into the thoracic cavity.
The cancer cells reach the pleura containing a small amount of pleural fluid.
These cancer cells&#39; receptors bind to specific ECM polysaccharides produced by the mesothelial cells lining the pleura, facilitating cell attachment to the pleural surface.&#160;
The cancer cells process these polysaccharides, causing increased cancer cell migration toward the surface. They also release proteolytic enzymes and autocrine growth factors.
Proteolytic enzymes degrade the ECM proteins, promoting cancer cell invasion.
The absence of a functional immune system and the presence of autocrine growth factors, allow the unrestricted proliferation of the cancer cells, forming tumor nodules.
Over time, tumor nodules grow and disseminate within the pleural cavity, disrupting the normal physiological fluid balance.
This results in fluid accumulation in the pleural cavity, a condition known as pleural effusion.