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Encyclopedia of Experiments

Quantification of Tear Volume in an Autoimmune Dry Eye Rat Model using the Phenol Red Thread

This video demonstrates the phenol red thread test to determine tear volume in an autoimmune dry eye rat model. The pH-sensitive acidic phenol red thread changes color when it comes in contact with the alkaline tears. The shorter length of color change in the phenol red thread corresponds to reduced tear volume.

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Assessment of Dry Eye Features

NOTE: The anesthetized rats should be held gently by a gloved hand in an upright position on a flat surface to avoid movement.

  1. Measure the tear volume with phenol red thread.
  1. Use one pair of.......

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