JoVE Encyclopedia of Experiments
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Encyclopedia of Experiments
Quantification of Tear Volume in an Autoimmune Dry Eye Rat Model using the Phenol Red Thread
Overview
This video demonstrates the phenol red thread test to determine tear volume in an autoimmune dry eye rat model. The pH-sensitive acidic phenol red thread changes color when it comes in contact with the alkaline tears. The shorter length of color change in the phenol red thread corresponds to reduced tear volume.
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Source: Hou, A. et al., A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue. J. Vis. Exp. (2017)
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