eoe sub articles

EoE Sub Articles

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Preparation of Lacrimal Gland Extract
NOTE: Rats were anesthetized with the intraperitoneal injection of ketamine (75 mg/kg) and xylazine (10 mg/kg). Proper anesthetization was confirmed by toe pinching and tail pinching. Ophthalmic gel was applied to the rat&#39;s eyes to prevent dryness ...

assessment of cornea and tear smoothness in an autoimmune dry eye rat model

Source: Hou, A. et al., <a href="https://app.jove.com/v/55592">A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue.</a>&#160;J. Vis. Exp. (2017)
This video demonstrates a method for assessing corneal smoothness and tear film stability in an autoimmune rat model. It highlights the impact of gland dysfunction on tear film stability, crucial for understanding dry eye disease.

Assessment of Cornea and Tear Smoothness in an Autoimmune Dry Eye Rat Model

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. ...

The tear film covering the cornea consists of lipid, water, and mucin produced by various secretory glands. Dysfunctioning of lacrimal and meibomian glands affects tear film stability and cornea smoothness, resulting in dry eye disease.
To measure cornea and tear smoothness, position an anesthetized autoimmune rat model under a stereomicroscope equipped with a ring illuminator featuring evenly spaced LED&#160; lights encircling the objective lens.
Moisten the ocular surface with saline and spread it evenly for accurate smoothness assessment.
Focus the ring illuminator on the cornea&#39;s center, generating an illuminator ring image with two circular rows of dots.
In the unaffected control rat, the illuminator ring image on the ocular surface is round with evenly spaced dots, indicating a smooth cornea with a stable tear film.
Conversely, the distorted image of an autoimmune rat model indicates reduced corneal smoothness and unstable tear film, confirming dry eye disease.