JoVE Logo
Faculty Resource Center

Sign In

JoVE Encyclopedia of Experiments

Overview

Protocol

Encyclopedia of Experiments

Reporter Enzyme Fluorescence Imaging for In Vivo Tracking of Bacterial Infection

This video demonstrates the reporter enzyme fluorescence (REF) imaging for in vivo detection of Mycobacterium tuberculosis infection. The infected mice are intraperitoneally injected with a substrate for REF imaging. Upon being hydrolyzed by bacterial β-lactamase enzyme, the substrate emits fluorescence, the detection of which aids evaluation of infection.

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Strains and culture conditions

Note: M. tuberculosis strain CDC1551 is used in this study, but any M. tuberculosis strain can be used in the same manner.

  1. Grow the bacteria in M-OADC-TW (7H9 broth supplemented with 0.5% glycerol, 10% oleic acid dextrose compl.......

Log in or to access full content. Learn more about your institution’s access to JoVE content here

This article has been published

Video Coming Soon

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved