JoVE Encyclopedia of Experiments
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Encyclopedia of Experiments
This video demonstrates a method for assessing the intracellular growth of GFP-tagged Coxiella mutants within cultured epithelial cells. The mutant phenotype is visualized using immunostaining and epifluorescence microscopy, allowing the study of the effect of the mutation on intracellular growth.
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Source: Martinez, E. et al., Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants. J. Vis. Exp. (2015)
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