eoe sub articles

EoE Sub Articles

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Expression and purification of antibodies
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	<li>Maintenance of Chinese hamster ovary (CHO) cells
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		<li>Grow CHO cells in FreeStyle CHO Media supplemented with commercially available 1x glutamine supplement at 37 &#730;C shaking at 130 rpm with 5% CO2 using delong Erlenmeyer flasks either glass or disposable.<...

a technique to assess the in vivo localization of tumor-specific antibodies

Source: Shivange, G., et al. <a href="https://app.jove.com/v/60727">Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody.</a> J. Vis. Exp. (2020).
This video demonstrates a technique to study the in vivo localization of tumor-specific antibodies in a mouse tumor xenograft model. Upon subcutaneously injecting cancer cells into mice for tumor formation, fluorescently-labeled antibodies are injected that bind to tumor-specific receptors, enabling in vivo assessment of antibody localization via imaging.

A Technique to Assess the In Vivo Localization of Tumor-Specific Antibodies

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. ...

Take a human ovarian cancer cell suspension. Add a basement membrane matrix medium, and keep on ice to avoid gel formation.
Load the mixture into a syringe and inject subcutaneously into an athymic nude mouse. Remove the needle once the matrix undergoes gelation, preventing mixture expulsion.
The lack of an immune response in the immunodeficient mouse preserves the viability of the injected cells. The gel&#39;s extracellular matrix proteins and growth factors provide growth-promoting signals, facilitating tumor formation.
Tumor cells overexpress surface-bound folate receptor alpha-1, or FOLR1.
Take FOLR1-specific antibodies. Add a fluorescent dye and incubate, allowing dye conjugation with the antibodies.
Intravenously inject the labeled antibodies into the anesthetized tumor-bearing mouse.
Antibodies reach the tumor via circulation and bind to FOLR1 on tumor cells, fluorescently labeling the cell surface.
Perform in vivo fluorescence imaging. Upon excitation, the dye emits fluorescence, facilitating the assessment of antibody localization in the tumor.