JoVE Encyclopedia of Experiments
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Overview
Encyclopedia of Experiments
Flow Cytometry Staining of Mycobacterium tuberculosis Infected Macrophage Subpopulations
Overview
This video showcases a flow cytometric gating strategy utilizing a multi-color panel to analyze cell viability and specific fluorescent-conjugated anti-human antibody markers. This approach makes it possible to determine the percentage of Mycobacterium tuberculosis-infected cell populations accurately.
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Source: Mily, A., et al. Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection. J. Vis. Exp. (2020)
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